Subunit Composition and Partial Reactions of the 2-Oxoglutarate Dehydrogenase Complex of Acetobacter xylinum
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منابع مشابه
a+Ketoglutarate Dehydrogenase Complex of Acetobacter xylinum
The cu-ketoglutarate dehydrogenase complex of Acetobacterxylinum was purified to homogeneity. It consists of three main polypeptide chains with a total molecular weight of about 2.4 x 106. It catalyzes the overall Mg*+ and thiamin pyrophosphate-dependent, NAD+and CoA-linked oxidative decarboxylation of cY-ketoglutarate, as well as the partial reactions characteristic of the three enzyme compone...
متن کاملProperties and subunit composition of the pig heart 2-oxoglutarate dehydrogenase.
The 2-oxoglutarate dehydrogenase, one of the component enzymes of the 2-oxoglutarate dehydrogenase complex, has been highly purified. The enzyme has a sedimentation coefficient (s&~) of 10.3 S and diffusion coefficient (&o,~) of 3.92 X lo-’ cm2 s-l. The weight average molecular weight was estimated to be about 216,000 from the sedimentation equilibrium data. The content of right-handed LY helix...
متن کاملRegulatory Properties of the a-Ketoglutarate Dehydrogenase Complex of Acetobacter xylinum
The initial velocity studies on the a-ketoglutarate dehydrogenase complex in toluene/Triton X-lOO-permeabilized Acetobwter xylinum cells revealed that the regulatory properties of the complex, in situ, are the same as for the purified complex. Thus, sigmoidal kinetics with respect to a-ketoglutarate were obtained (So.5 (a-ketogkltarate) = 5 lfl~ ; nH = 1.8), which convert into a hyperbolic rela...
متن کاملFlavine Adenine Dinucleotide-linked Malic Dehydrogenase from Acetobacter Xylinum.
Benziman, Moshe (The Hebrew University of Jerusalem, Jerusalem, Israel), and Y. Galanter. Flavine adenine dinucleotide-linked malic dehydrogenase from Acetobacter xylinum. J. Bacteriol. 88:1010-1018. 1964.-The properties of the pyridine nucleotide-nonlinked malic dehydrogenase of Acetobacter xylinum were investigated in the supernatant fluid obtained by high-speed centrifugation of sonic extrac...
متن کاملSynthesis of Cellulose by Acetobacter Xylinum
Appearance of cellulose microfibrils in the medium of a suspension of cells of Acetobacter xylinum in buffered glucose solution was preceded by a stage during which the cellulose in the medium was amorphous within the available resolution. The size of the vertical axis of the microfibrils of the bacterial cellulose was found on the basis of measurement of shadow length to be only about 16 A. In...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2005
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1980.tb05996.x